TABLE 1.
Gradient flow rate and relative composition of eluents used for HPLC determination of nucleic acids at a gradient flow rate of 1.6 ml/min
| Time (min) | Composition (%)a
|
||
|---|---|---|---|
| A | B | C | |
| 0 | 0 | 0 | 100 |
| 0.32 | 0 | 0 | 100 |
| 0.63 | 85 | 15 | 0 |
| 6.25 | 85 | 15 | 0 |
| 18.75 | 0 | 100 | 0 |
| 18.95 | 0 | 100 | 0 |
| 19.00 | 0 | 0 | 100 |
| 23.00 | 0 | 0 | 100 |
a
A, 300 g of urea, 1.361 g of KH2PO4, and 1.742 g of K2HPO4 in 1 liter of MilliQ water at pH 6.7 with H3PO4; B, 300 g of urea, 112 g of KCl, 1.361 g of KH2PO4, and 1.742 g of K2HPO4 in 1 liter of MilliQ water at pH 6.7 with H3PO4; C, 0.01% sodium azide in MilliQ water.