TABLE 2.
DNA and RNA concentrations and RNA/DNA ratios at different depths
| Depth (m) | Comparison of methodsa
|
Fluorometric DNAb
|
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|---|---|---|---|---|---|---|---|---|---|---|---|
| a
|
b
|
c
|
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| Mean concn ± SD (μg g−1)
|
Mean concn ± SD (μg g−1)
|
Mean concn ± SD (μg g−1)
|
|||||||||
| DNA | RNA | RNA/DNA | DNA | RNA | RNA/DNA | DNA | RNA | RNA/DNA | F/S | F/H | |
| 40 | 39.7 ± 14.2 | 29.9 ± 5 | 0.84 | 18.6 ± 0.3 | 5.7 ± 2.8 | 0.31 | 39.8 ± 4.4 | 24.1 ± 2.4 | 0.61 | 47 | 47 |
| 100 | 38.5 ± 11 | 19.1 ± 3.1 | 0.96 | 31.4 ± 1.9 | 25.0 ± 3.5 | 0.8 | 37.3 ± 0.5 | 9.7 ± 0.4 | 0.26 | 82 | 84 |
| 200 | 37.4 ± 19.3 | 20.5 ± 13.5 | 0.58 | 5.1 ± 0.4 | 5.3 ± 1 | 1.04 | 34.5 ± 3.1 | 6 ± 0.3 | 0.17 | 14 | 15 |
| 500 | 16.6 ± 4.7 | 5.1 ± 0.9 | 0.157 | 2.9 ± 0.1 | 3.6 ± 0.3 | 1.25 | 31.7 ± 3.3 | 2.9 ± 0.2 | 0.09 | 17 | 9 |
| 700 | 34 ± 5.8 | 2.2 ± 1.1 | 0.05 | 8.4 ± 0.7 | 7.5 ± 1.9 | 0.9 | 33.5 ± 2.2 | 2.8 ± 0.2 | 0.08 | 25 | 25 |
| 900 | 37.7 ± 10.4 | 0.8 ± 0.4 | 0.004 | 3.7 ± 0.4 | 6.7 ± 1.9 | 1.82 | 25.2 ± 4.3 | 2.7 ± 0.2 | 0.11 | 10 | 15 |
| 1,540 | 12.3 ± 8.2 | 0.4 ± 0.7 | 0.08 | 2.1 ± 0.5 | 4.7 ± 1.4 | 2.2 | 33.6 ± 1.2 | 1.3 ± 0.2 | 0.04 | 17 | 6 |
| Avg | 30.9 | 11.1 | 10.3 | 8.4 | 33.7 | 7.1 | 30 | 29 | |||
a
ANOVA results of DNA determinations by spectrophotometric (a), fluorometric (b), and HPLC (c) methods: a versus b, P < 0.01; a versus c, not significant; b versus c, P < 0.01. ANOVA results for RNA determinations: a versus b, not significant; a versus c, not significant at 40-m depth and below 200-m depth; b versus c, P < 0.05.
b
Contribution of fluorometric DNA concentrations (as percentage) to the spectrophotometric (F/S) and HPLC (F/H) DNA concentrations at different depths.