Figure 7.

GSL levels were higher in female-derived compared to male-derived hRMCs. LacCers (A) and HexCers (B) were measured in female-derived and male-derived hRMCs and normalized to cell viability. The major chain lengths expressed in the hRMCs and the total of all chain lengths are shown in the graphs. The vehicle-treated female- or male-derived cells from the respective experiments presented in Figure 5 were scraped and combined to measure GLSs. GlcCers and LacCers levels were normalized to the cell viability determined prior to scraping. (C) N-glycans were measured by MALDI-FTICR in female- or male-derived hRMCs plated in duplicate. Individual glycans are presented as the relative peak intensity of the total glycans measured in the individual wells. Means + SD shown on graphs. Statistical analyses were not performed for the GSL or glycan analyses since the measures were performed in hRMCs from only one female donor and one male donor.