TABLE 1.
Purification of bacteriocins from L. sake LTH673 and assessment of yields with various indicator strains
| Indicator strain | Total activity, 10−5 bacteriocin units, of:
|
Yield (%)b | |
|---|---|---|---|
| Culture supernatant | Purified sakacin Pa | ||
| Lactobacillus coryneformis NCDO 2740 | 6.6 | 3.4 | 52 |
| L. sake NCDO 2714 | 76 | 4.1 | 5.4 |
| Lactobacillus sp. strain LMG 2804 | 8.5 | 0.09 | 1.1 |
a
Ten steps of Edman degradation were performed and indicated that the material contained >98% pure sakacin P.
b
The dramatic indicator strain-dependent variation in yield showed that antimicrobial activity toward some strains had been lost, whereas activity toward other strains had been retained (see the text for details).