TABLE 3.
Effect of reduction on antimicrobial activitya
| Strain | Sensitivity to:
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Pediocin PA-1
|
Enterocin A
|
Curvacin A
|
Sakacin P
|
|||||
| Standard | + DTT | Standard | + DTT | Standard | + DTT | Standard | + DTT | |
| L. sake NCDO 2714 | 0.4 | 45 (0.9) | 0.1 | 19 (0.5) | 1.1 | 4.5 (24) | 3.7 | 29 (13) |
| Lactobacillus sp. strain LMG 2333 | 0.8 | 8.2 × 102 (0.1) | 1.6 | 1.9 × 102 (0.9) | 4.0 | 48 (8.3) | 8.6 | 54 (16) |
| P. pentosaceus LMG 2722 | 0.4 | 3.2 × 102 (0.1) | 0.5 | 84 (0.6) | 5.4 × 102 | >20 × 102 (<27) | 1.5 × 102 | 5.4 × 102 (28) |
a
The standard microtiter plate assay was used, with or without the addition of 10 mM DTT (final concentration) to each well. No growth inhibition occurred in control cultures without bacteriocin but with 10 mM DTT. Sensitivity is reported as MIC in nanograms per milliliter. Values in parentheses indicate the percentages of antimicrobial activity under reducing conditions relative to that under standard (nonreducing) conditions.