sdAb	Single-domain antibody
hcAb	Heavy-chain antibody
VHH	Variable domain of the heavy chain antibody
VNAR	Variable domain of new antigen receptor
CDR	Complementarity determining region
HV2	Hypervariable region 2
HV4	Hypervariable region 4
scFv	Single-chain variable fragment
NGS	Next generation sequencing
N3	The phage pool in Round 3 under normal condition
L3	The phage pool in Round 3 under low-temperature condition
H3	The phage pool in Round 3 under preheat condition
S3	The phage pool in Round 3 under high salt concentration condition
U3	The phage pool in Round 3 under high urea concentration condition
ITC	Isothermal titration calorimetry
DSC	Differential scanning calorimetry
SPR	Surface plasmon resonance
kon	Association rate constant
koff	Dissociation rate constant
Tm	Melting Temperature
ΔG	Gibbs free energy change
ΔH	Enthalpy change
ΔS	Entropy change
ΔCp	The change in heat capacity