(A) Disease course of EAE Cpeb3fl/fl;Snap25-Cre mice (n = 12) and Cpeb3fl/fl littermate controls (n = 17) showing mean clinical disability of pooled data from two independent experiments. Mann-Whitney U test of the area under the curve (AUC) of each animal, P = 0.0018. (B) Immunohistochemical staining and quantification of CD3+ cells in cervical spinal cords of Cpeb3fl/fl;Snap25-Cre versus Cpeb3fl/fl control mice at EAE day 30 after immunization. CD3+ cells are normalized to the spinal cord area. Mann-Whitney U test, P > 0.9999, n = 5. Scale bar, 100 μm. (C) Immunohistochemical staining and quantification of the IBA+ area in cervical spinal cord of Cpeb3fl/fl;Snap25-Cre versus Cpeb3fl/fl control mice at EAE day 30 after immunization. Mann-Whitney U test, P > 0.3905, n = 5. Scale bar, 100 μm. (D) Staining of myelin with Luxol-Fast-Blue (LFB) in cervical spinal cords of Cpeb3fl/fl;Snap25-Cre versus Cpeb3fl/fl control mice at EAE day 30 after immunization. The LFB+ area is normalized to the spinal cord area. Student’s t test, P = 0.8413, n = 5. Scale bar, 250 μm. (E) Immunohistochemical staining and quantification of injured APP+ axons in cervical spinal cord white matter of Cpeb3fl/fl;Snap25-Cre versus Cpeb3fl/fl control mice at EAE day 30 after immunization. Mann-Whitney U test, P = 0.0159, n = 5. Scale bar, 100 μm.