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. 2023 Nov 24;9(47):eadf2772. doi: 10.1126/sciadv.adf2772

Fig. 5. NANS mutation disrupts VZ integrity and disturbs corticogenesis in cerebral organoids.

Fig. 5.

(A) UMAP plots and pie charts comparing NPCs, upper-layer neurons, and deep-layer neurons between WT and NANS-KO cerebral organoids. Numbers indicate the percentage of total cells belonging to the respective cell type. (B) Representative images of the VZ-like area in WT and NANS-KO cerebral organoids. Scale bar, 50 μm. (C) Quantification of the loop area of VZ, oSVZ, and CP. n = 45 to 48, ***P < 0.001, Student’s t test. (D) Representative images of WT and NANS-KO cerebral organoids after staining with DAPI, SOX2, TUJ1, and CTIP2. Scale bar, 10 μm. (E) Statistical analysis of marker distribution across the VZ/oSVZ/CP within organized regions in WT and NANS-KO cerebral organoids. n = 9 to 10, *P < 0.05, **P < 0.01, ***P < 0.001, two-way repeated-measure ANOVA followed by the Bonferroni’s multiple comparisons test.