Figure 4.

Cell wall remodeling in P. lutzii under proline conditions. Yeast cells were collected after 48 h of culture under NCR conditions, stained with aniline blue (AB) and calcofluor white (CFW) and visualized using an Axiocam MRc-Scope A1 fluorescence microscope. (a) Cells stained with AB in NCR-glutamine and NCR-proline conditions. (b) Fluorescence intensity of AB (in pixels) for yeast cells under NCR conditions. (c) Cells stained with CFW under NCR-glutamine and NCR-proline conditions. (d) Fluorescence intensity of CFW (in pixels) for yeast cells under NCR conditions was determined using AxioVision Software, determining the standard error in each analysis. Statistical comparisons were performed using Student’s t-test, with p ≤ 0.0001 (****), considered statistically significant. Prol: proline; Glut: glutamine; Magnification 40×.