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. 2023 Nov 12;9(11):1102. doi: 10.3390/jof9111102

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Transcriptional level of NCR-related genes under NCR conditions. The expression of NCR-related genes in P. lutzii yeast cells under NCR conditions was assessed through RT-qPCR, using relative quantification. The tubulin gene (PAAG_03031) served as the endogenous control, and the transcriptional levels of the genes coding for γ-glutamyl transpeptidase (ggt), formamidase (fmd), arginase (arg) and urease (ure) were analyzed. Data are expressed as the mean ± standard deviation of triplicates of independent experiments. Statistical analysis was performed through the Student’s t-test, demonstrating values of p ≤ 0.05 (*), p ≤ 0.01 (**) and p ≤ 0.001 (***), considered as statistically significant. P: proline; G: glutamine.