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. 2023 Nov 12;9(11):1102. doi: 10.3390/jof9111102

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Enzymatic activity of enzymes related to cell rescue and virulence under NCR conditions. The assays were performed using soluble proteins obtained under NCR conditions. (a) The enzymatic activity of gamma-glutamyltransferase (γ-GT or GGT) was assessed using 4 μg and 12 μg of soluble proteins from NCR-proline and NCR-glutamine conditions. GGT specific activity (U/L) was calculated by measuring the number of nmol of ρNA released per liter of sample in the incubation time (min). (b) GST activity was measured using a total of 10 μg of soluble proteins obtained in NCR-proline and NCR-glutamine conditions. Student’s t-test was applied for statistical analysis, showing values of p ≤ 0.01 (**), p ≤ 0.001 (***) and p ≤ 0.0001 (****), considered as statistically significant. Prol: proline; Glut: glutamine.