FIG. 6.

Amino acid sequence alignment of LAMY, strain 707 enzyme, BAA, BSA, and BLA. Each numbering starts after the respective signal peptide. The four conserved regions, I, II, III, and IV (30), are boxed. Domains A and B are indicated by dotted boxes and shading, respectively. The remaining C-terminal sequence corresponds to domain C. Underlined sequences correspond to the secondary structure elements α1 to α8 and β1 to β8 of the BLA (α/β)₈ barrel (domain A) according to Machius et al. (24). BLA residues involved in the catalytic site (★), calcium binding site (○), and chloride binding site (•) are indicated above the sequences. Sequence accession numbers (Swiss-Prot database) were as follows: strain 707 enzyme, P19571; BAA, P00692; BSA, P06279; and BLA, P06278.