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Purification of LAMY produced by Bacillus sp. strain KSM-1378
| Purification step | Total amt of protein (mg) | Total activity (U) | Sp act (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
| Culture filtrate | 353.6 | 292,401 | 827.0 | 100 | 1.0 |
| 60% Ammonium sulfate precipitation | 68.8 | 399,572 | 3,811.9 | 137 | 4.6 |
| DEAE-Toyopearl, unadsorbed | 55.4 | 218,067 | 3,935.3 | 75 | 4.8 |
| CM-Toyopearl chromatography | 20.4 | 102,039 | 5,009.3 | 35 | 6.1 |
