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. 2023 Nov 4;9(11):1078. doi: 10.3390/jof9111078

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Formation of C. albicans SC5314 biofilm and its disruption in the presence of biofilm-derived EVs produced by C. albicans strains 3147 and SC5314. The biofilm thickness was investigated by measuring OD at 600 nm in the area scan mode (A) before the washing step (biofilm formation) and after washing (biofilm detachment), and by crystal violet staining (B). A combined result of four independent experiments is presented. The statistical significance levels compared to biofilm cultured without EVs were marked with ^# for p < 0.05, ^## for p < 0.01, ^#### for p < 0.0001, and compared to the biofilm before the washing step with ** for p < 0.01, **** for p < 0.0001, and ns when not significant.