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. 2023 Oct 25;21(11):553. doi: 10.3390/md21110553

Figure 4.

Figure 4

The anti-inflammatory effects of 1 in RAW264.7 cells. (A) NO production in RAW264.7 cells measured after 24 h using Griess reagent. The cells were pretreated with 1 at different concentrations for 1 h followed by stimulation with LPS (1 µg/mL). (B) Cell viability in RAW264.7 after 24 h treatment with 1 measured using MTT assay. (C,D) Relative transcript levels of Nqo1 and iNos measured by TaqMan analysis, using actin as endogenous control. RAW264.7 cells were pretreated with compound 1 at different concentrations for 1 h followed by LPS stimulation (1 µg/mL). RNA was extracted following 12 h incubation. Data represent mean ± SD (n = 3). The asterisks denote significance of p < 0.05 relative to DMSO + LPS using two-tailed unpaired t-test (** denotes p ≤ 0.01, *** denotes p ≤ 0.001, **** denotes p ≤ 0.0001).