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Sodium dodecyl sulfate-PAGE analysis of different stages during the purification of MGL from B. linens BL2 of fractions containing enzyme activity. Lanes: 1, CE; 2, 55 to 65% ammonium sulfate fraction; 3, DEAE-Fractogel column; 4, hydrophobic-interaction column; 5, Mono Q; 6, Superose 12; 7, molecular mass marker proteins. Approximate molecular masses in kilodaltons (K) are indicated on the right.
