Table 4.
Analysis of variance (ANOVA) for the mixture design and regression equation for the response variables (Bacterial cycle changes after mixture and under gastric pH and salt bile conditions).
| Source | Change in Bacterial Cycles | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| After Mixture | Gastric pH | Bile Salts | |||||||||||||
| SS * | Df ** | Mean Square | F-Value | p-Value | SS * | Df ** | Mean Square | F-Value | p-Value | SS * | Df ** | Mean Square | F-Value | p-Value | |
| Model | 0.09 | 4 | 0.02 | 34.5 | 0.0008 | 14.6 | 2 | 7.29 | 27.6 | 0.0005 | 1.39 | 2 | 0.69 | 10.4 | 0.0080 |
| Linear mixture |
6.19 × 10−4 | 1 | 6.19 × 10−4 | 0.95 | 0.3740 | 0.280 | 1 | 0.280 | 1.05 | 0.3399 | 0.59 | 1 | 0.59 | 8.89 | 0.0204 |
| AB | 9.07 × 10−3 | 1 | 9.07 × 10−3 | 13.9 | 0.0135 | 14.3 | 1 | 14.3 | 54.1 | 0.0002 | 0.80 | 1 | 0.80 | 11.90 | 0.0107 |
| AB(A-B) | 2.25 × 10−3 | 1 | 2.25 × 10−3 | 3.46 | 0.1220 | ||||||||||
| AB(A-B)2 | 0.02 | 1 | 0.023 | 34.7 | 0.0020 | ||||||||||
| Residual | 3.25 × 10−3 | 5 | 6.50 × 10−4 | 1.85 | 7 | 0.260 | 0.47 | 7 | 0.07 | ||||||
| Lack of fit | 2.60 × 10−3 | 2 | 1.30 × 10−3 | 6.00 | 0.0894 | 1.01 | 4 | 0.250 | 0.91 | 0.5543 | 0.29 | 4 | 0.07 | 1.28 | 0.4376 |
| Pure error | 6.50 × 10−4 | 3 | 2.17 × 10−4 | 0.84 | 3 | 0.280 | 0.17 | 3 | 0.06 | ||||||
| Corr. Total | 0.09 | 9 | 16.43 | 9 | 1.86 | 9 | |||||||||
| R2 | 0.96 | 0.88 | 0.74 | ||||||||||||
| Equation | N (log CFU/mL) = −0.045358 ∗ A − 0.040809 ∗ B + 1.73313 × 10−3 ∗ A ∗ B + 1.39291 × 10−6 ∗ B ∗ A ∗ (A − B) + 2.54879 × 10−7 ∗ A∗ B ∗ (A − B)2 | N (log CFU/mL Log) = −0.070443 ∗ A − 0.084910 ∗ B + 3.09535 × 10−3 ∗ A ∗ B | N (log CFU/mL) = 0.024460 ∗ A − 0.036966 ∗ B + 7.29884 × 10−4 ∗ A ∗ B |
||||||||||||
A: Gelatin, B: Culture, * Sum of squares, ** Degrees of freedom.