Figure 3.

Expression of LMP1 mRNA in EBV-positive T- or NK-cell lines and in the stable transformants of human T-cell lines transfected with pEBMulti-Neo-LMP1 alone or both pEBMulti-Neo-EKS6 and pEBMulti-Neo-LMP1. Real-time RT-PCR specific for LMP1 mRNA was performed as described in Materials and Methods Section. Bars indicate the relative expression level of mRNA compared to that in TK6 cells, which was standardized with the expression of mRNA of glyceraldehyde-3-phosphate dehydrogenase, represented as permillage of that in TK6 cells (LCLs).