Table 2.

The effect of propolis ethanolic extract on the production of selected cytokines, i.e. IL-1β, IL-6, IL-8, IL-15, TNF-α and EEP in LPS, EEP in IFN-α, as well as EEP in LPS + IFN-α stimulated HGF-1 cells (n = 3). Statistical significance was analyzed using Fisher’s LSD test. Results marked in bold are statistically significant in Fisher’s LSD test. Multivariate tests of significance (F = 12.91, p < 0.05).

Sample	IL-1β			IL-6			IL-8			IL-15			TNF-α
	AVG	SD	p	AVG	SD	p	AVG	SD	p	AVG	SD	p	AVG	SD	p
Control baseline (EEP)	1.224			3.410			1.360			1256.310			4.070
Control DMSO (EI)	3.261	1.778		109.283	8.940		27.600	6.946		1482.840	156.863		11.330	7.335
Control LPS (ELI)	1.315	0.079		131.800	23.512		41.693	4.151		1439.930	373.413		3.642	0.214
Control IFN-α (EI)	2.153	1.374		105.797	21.038		24.810	5.595		1730.793	161.382		7.222	6.018
Control LPS + IFN-α (ELI)	1.156	0.068		139.400	27.655		27.477	4.218		1534.390	32.684		3.427	0.742
EEP 25 µg/mL	3.603	2.178		87.747	33.217		42.423	1.686		1505.647	44.126		7.294	5.959
EEP 50 µg/mL	1.315	0.079		114.503	25.121		40.233	6.905		1539.677	248.184		3.642	0.214
EEP 25 µg/mL + LPS	1.224	0.000	0.9442	85.727	35.643	0.9941	48.643	13.891	0.9889	1450.367	116.122	0.9178	3.856	0.214	0.9736
EEP 50 µg/mL + LPS	1.088	0.118	0.8611	143.770	67.882	0.9985	87.427	53.326	0.9271	1470.890	87.826	0.7596	5.251	2.416	0.8040
EEP 25 µg/mL + IFN-α	1.315	0.079	0.5182	392.797	35.264	0.9633	608.307	204.011	0.2460	1711.937	53.431	0.8521	4.965	2.663	0.7277
EEP 50 µg/mL + IFN-α	1.269	0.079	0.4960	371.620	27.279	0.9660	422.833	164.744	0.4271	1386.310	86.914	0.0012	3.356	0.539	0.5514
EEP 25 µg/mL + LPS + IFN-α	1.156	0.118	1.0000	408.203	35.695	0.9656	870.680	139.463	0.0959	1483.853	134.711	0.6177	7.080	6.164	0.5736
EEP 50 µg/mL + LPS + IFN-α	2.811	1.610	0.2050	449.213	54.085	0.9604	1209.220	246.153	0.0212	1581.167	42.413	0.6440	10.259	8.171	0.2943

AVG: average; SD: standard deviation; p-value: probability value.