‘Honeycrisp’ apples co-inoculated with P. chrysogenum and P. expansum isolates at different times: (A) Mean lesion diameter of wounds inoculated with P. expansum R19 and Pe21 at (left to right) 0, 3, and 7 days after P. chrysogenum 404 and 413 inoculation into wounded ‘Honeycrisp’ apples (0, 3, and 7 DBI). All measurements were taken at 3, 5, and 7 days (D3, D5, and D7) postinoculation of the P. expansum inoculum, with the mean wound diameter of TTW-inoculated fruit subtracted from each strain set. Data are presented from a representative trial, and error bars represent the standard deviation for each trial (n = 5). An ANOVA test with Tukey’s HSD post hoc test (p < 0.05) or a Kruskal–Wallis test with Wilcoxon post hoc test (p < 0.05) was performed to determine the significance between strains at each timepoint. Values with the same letter are not significantly different. (B) Representative photos of lesions formed 7 days after inoculation of R19 (left) or Pe21 (right) into wound 0, 3, and 7 days (0, 3, 7 DBI) after initial inoculation of (top to bottom) TTW control, 404, or 413. (C) Percent incidence of rot observed 7 days after P. expansum inoculation. The percent incidence shown is from both trials at the 7 DBI timepoint. DBI = days between inoculations, TTW = tween-treated water.