Table 1.

Linear regression data from a six-point calibration curve and parameters of method validation.

SCFA	Retention Time (min)	Slope (m)	Intercept (b)	R2	LOD (µg/mL)	LOQ (µg/mL)	Intra-Day at 2000 ppm (% RSD)	Inter-Day at 50 ppm (% RSD)	Inter-Day at 750 ppm (% RSD)	Inter-Day at 2000 ppm (% RSD)
Acetic	3.32	0.04	0.002	0.99994	0.15	0.50	0.66	4.76	0.92	0.35
Propionic	3.74	0.61	0.001	0.99996	0.17	0.56	0.64	0.85	1.52	0.93
IsoButyric	3.91	0.08	0.002	0.99995	0.23	0.78	N/A	N/A	N/A	N/A
Butyric	4.20	0.08	0.001	0.99996	0.10	0.32	0.77	0.45	1.11	0.78
IsoValeric	4.42	0.09	0.001	0.99997	0.07	0.23	0.56	0.81	0.10	0.23
Valeric	4.78	0.91	0.000	0.99998	0.06	0.21	1.03	0.99	0.86	0.64
4-methyl valeric	5.12	0.09	0.001	0.99998	0.04	0.13	0.82	0.73	0.44	0.74
Hexanoic	5.34	1.02	0.001	0.99998	0.04	0.12	0.82	0.80	0.35	0.18
Heptanoic	5.89	1.05	0.003	0.99998	0.02	0.08	0.69	1.11	0.36	0.63

LOD—limit of detection; LOQ—limit of quantification; RSD—relative standard deviation; N/A—not available; SCFA—short-chain fatty acid.