Table 3.

Comparison of SCFAs extraction methods from fresh fecal samples.

SCFAs	AE Method Amount (µmol/g) 1	AE Method % RSD	TBME Method Amount (µmol/g) 1	TBME Method % RSD	p-Value AE vs. TBME 2,3
Acetic	69.42	8.63	36.80	20.94	<0.0001
Propionic	20.95	8.82	12.57	19.81	<0.0001
IsoButyric	5.89	6.82	3.53	19.45	<0.0001
Butyric	22.25	2.49	13.81	19.09	<0.0001
IsoValeric	8.48	6.05	5.24	20.21	<0.0001
Valeric	5.44	2.18	3.21	19.62	<0.0001
4-methyl valeric	ND	N/A	ND	N/A	N/A
Hexanoic	0.57	2.15	0.25	17.98	<0.0001
Heptanoic	ND	N/A	ND	N/A	N/A

n = 3; n—number of samples prepared by each extraction method. ¹ Values are unadjusted means. ² Student’s t-test. ³ Statistical significance at p < 0.05. SCFAs—short-chain fatty acids; AE—aqueous extraction; TBME—tert-butyl methyl ether extraction; RSD—relative standard deviation; ND—not detected; N/A—not applicable.