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. 2023 Nov 19;11(11):2805. doi: 10.3390/microorganisms11112805

Figure 3.

Figure 3

Configuration of DNA arrays used in CARD cartridge. Probes against Mit1C and IAC were spotted on the DNA array at concentrations ranging from 1.25 µM to 80 µM, as noted. Spots designated as RS1, RS9, and RS19 represent an 18mer biotinylated oligo dT, which is used as one of the controls to demonstrate the horseradish peroxidase, TMB reaction performed correctly. Regardless of the presence of any of the target hybridization spots, the RS spots must be present for the assay to be considered valid.