Table 4.
Thermocycling conditions used in the Encompass Optimum workstation.
| PCR Step | Temperature/Time/Number of Cycles |
|---|---|
| Initial denaturation | 96 °C/3 min/1 cycle |
| Heat denaturation | 96 °C/15 s/40 cycles |
| Annealing/elongation | 62 °C/1 min/40 cycles |
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