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. 1998 Sep;64(9):3352–3358. doi: 10.1128/aem.64.9.3352-3358.1998

TABLE 1.

Separation of phytoplankton and bacteria by prefiltrationa

Sample Positionb Sampling depth (m) Date Filter typec Chl. a
Cyano
Bacteria
Carbon ratio (%)e based on:
Concn in SW (μg liter−1) % in filtrated No. in SW (103 ml−1) % in filtrated No. in SW (105 ml−1) % in filtrated Chl. a data Cyano data
Oceanic
 Southern (south) 65°24′S, 140°40′E 40 18 June 1995 8AU 0.48 0.37 <0.03 f 2.9 93 1.4 <0.01
 Southern (north) 48°32′S, 146°23′E and 47°46′S, 146°41′E 4 25 June 1995 8AU 0.49 0.65 8.0 <0.4 6.0 96 4.3 <0.2
 Subtropical Pacific (south) 14°52′S, 155°10′E and 12°59′S, 154°53′E 4 4 Feb. 1995 8AU 0.078 17.8 0.97 <6 5.8 91 11 <2
 Subtropical Pacific (north) 15°20′N, 141°24′E and 16°27′N, 140°20′E 4 10 Feb. 1995 8AU 0.10 8.2 0.67 <10 6.6 87 5.6 <3
 Equatorial Pacific 0°02′S, 150°42′E and 1°35′N, 149°34′E 4 7 Feb. 1995 8AU 0.21 0.99 35 <0.2 8.3 92 2.3 <4
 Transitional north Pacific 31°20′N, 140°25′E and 32°22′N, 140°13′E 4 12 Feb. 1995 8AU 0.23 0.52 3.2 <3 5.4 94 0.90 <3
Coastal
 Tokyo Bay 35°08′N, 139°46′E 1 30 July 1996 8AU 11.8 0.32 61 81 0.93
 Otsuchi Bay 39°21′N, 141°59′E 2 2 June 1995 GF-75 7.2 0.26 23 2.7 21 67 3.0 1.1
39°21′N, 141°59′E 5 5 June 1995 GF-75 4.1 0.26 37 2.3 20 72 1.8 1.0
39°21′N, 141°59′E 10 17 May 1996 GF-75 4.2 4.7 33 37 86
39°21′N, 141°59′E 10 20 May 1996 GF-75 3.6 2.6 29 23 15
a

Comparisons of chlorophyll a (Chl. a) concentrations and cell abundances of cyanobacteria (Cyano) and bacteria are made between whole seawater (SW) and filtrates. 

b

Some samples were taken while the ship was steaming. In these cases, positions at the beginning and end of sampling are reported. 

c

8AU, Micropore 8AU; GF-75, Advantec GF-75. Nominal pore sizes are given in Materials and Methods. 

d

Percentage of the chlorophyll a concentration or the number of cells (cyanobacteria or bacteria) in whole seawater that passed through the prefiltration filter. 

e

Ratio of carbon in phytoplankton to total organic carbon in the concentrated samples; calculations were based on a carbon-to-chlorophyll a ratio of 50 and a cyanobacterial carbon content of 200 fg cell−1. Chlorophyll a concentrations and cyanobacterial abundances of concentrated oceanic samples were calculated from those in prefiltered samples by assuming that the concentration factors of chlorophyll a and cyanobacteria are the same as that of bacteria (see the text). 

f

—, not determined.