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. 1998 Sep;64(9):3352–3358. doi: 10.1128/aem.64.9.3352-3358.1998

TABLE 2.

Concentration of bacteria by tangential flow filtrationa

Sample Seawater
Bacteria
% of bacteria recovered in:
Initial vol (liters) Concn factor Initial no. (105 ml−1) No. in retentate (107 ml−1) Concn factor Retentateb Permeatec Retentate + permeated Ultraconcentrated samplee
Oceanic
 Southern (south) 95 103 2.7 0.39 14 14 32 46 11
 Southern (north) 300 268 5.7 13 225 84 12 96 16
 Subtropical Pacific (south) 448 446 5.3 1.3 24 5.3 7.3 13 4
 Subtropical Pacific (north) 550 546 5.8 1.8 31 5.6 68 74 1
 Equatorial Pacific 634 649 7.6 9.6 126 19 51 70 9
 Transitional north Pacific 320 307 5.0 0.69 14 4.5 NDf ND 3
Coastal
 Tokyo Bayg 50 95 49 11 21 22 31 53 18
 Otsuchi Bay
  2 June 1995 140 157 14 19 131 83 0.02 83 26
  5 June 1995 190 110 14 12 88 43 0.03 43 23
  17 May 1996 95 88 12 0.84 6.9 7.8 1.5 9.3 2
  20 May 1996 100 98 7.0 0.89 13 14 1.8 16 2
a

Bacterial cell abundances in prefiltered seawater (initial), the concentrated fraction (retentate), and the filtrate fraction (permeate) are compared. 

b

(Total number of cells in retentate/total number of cells in prefiltered seawater) × 100. 

c

(Total number of cells in permeate/total number of cells in prefiltered seawater) × 100. 

d

[(Total number of cells in retentate + total number of cells in permeate)/total number of cells in prefiltered seawater] × 100. 

e

(Total number of cells in the concentrate used for measuring carbon and nitrogen/total number of cells in raw seawater) × 100. 

f

ND, not determined. 

g

A hollow-fiber cartridge filtration system was used to concentrate bacteria.