Table 2.

ANOVA for quadratic model of the second response for the total number of peaks.

Source	Sum of Squares	df	Mean Square	F-Value	p-Value	Note
Model	39.68	13	3.05	164.03	<0.0001	significant
A-pH	0.0056	1	0.0056	0.3019	0.5899
B-Flow Rate	0.0018	1	0.0018	0.0965	0.7599
C-Temperature	1.24	1	1.24	66.82	<0.0001
D-Organic Phase	33.28	1	33.28	1788.17	<0.0001
AB	1.70	1	1.70	91.49	<0.0001
AC	0.0020	1	0.0020	0.1087	0.7457
AD	0.0019	1	0.0019	0.1018	0.7536
BC	0.0163	1	0.0163	0.8742	0.3629
BD	0.0053	1	0.0053	0.2850	0.6003
CD	0.7439	1	0.7439	39.97	<0.0001
A²	0.4545	1	0.4545	24.42	0.0001
B²	0.7120	1	0.7120	38.26	<0.0001
C²	2.43	1	2.43	130.52	<0.0001
Residual	0.3164	17	0.0186
Lack of Fit	0.3164	8	0.0395
Pure Error	0.0000	9	0.0000
Cor Total	40.00	30