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The peak areas of metabolite G-M5 during gentiopicrin incubation with rat intestinal bacteria for 0 min, 30 min, 60 min, 90 min, 120 min, and 180 min (A). The MS1 and MS2 spectra of metabolite G-M5 acquired by LC/MSn-IT-TOF (B). Possible structure and cleavage pathway of metabolite G-M5 determined by mass spectrometry (C).
