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. 2023 Nov 9;13(11):1139. doi: 10.3390/metabo13111139

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Altered metabolism between tumor and normal regions in murine glioma. (a) Wasserstein distance between m/z peak distributions of tumor and normal regions for each combination in small-molecule screen. (b) Wasserstein distance between m/z peak distributions of tumor and normal regions for each combination in lipid screen. (c) Normalized enrichment score for KEGG pathways for each combination and bulk RNA-seq data of tumor and normal regions for small-molecule screen. (d) Abundance of L-Arginine across tumor (red rectangle), border (yellow rectangle), and adjacent normal (green rectangle) as detected by recrystallized CHCA in positive polarity mode on the left and 9-AA in negative polarity mode on the right; the line plot underneath shows the average intensity across the different areas. (e) Three-dimensional plot of the abundance of L-Arginine for recrystallized CHCA matrix in positive polarity mode (top) and 9-AA matrix in negative polarity mode (bottom).