TABLE 2.
Viral parameters of sewage water samples
| Sample | PCR resulta
|
Enterovirus on cell cultureb | B. fragilis bacteriophagesc | ||
|---|---|---|---|---|---|
| HAd | HAV | EV | |||
| January | + | − | − | T | 34 |
| February | + | − | − | T | 41 |
| March | − | − | − | T | 25 |
| April | + | − | + | T | 49 |
| May | + | − | + | 14 | 209 |
| June | + | + | + | T | 34 |
| July | + | + | − | 0 | 85 |
| August | + | − | + | 0 | 160 |
| September | + | − | − | 0 | 44 |
| October | + | + | + | 0 | 33 |
| November | + | − | − | 0 | 62 |
| December | + | − | − | 0 | 74 |
| February | |||||
| Influent | + | + | + | 0 | 110 |
| Effluent | + | − | − | 0 | 63 |
| April | |||||
| Influent | + | − | − | 0 | 48 |
| Effluent | + | − | − | 0 | 139 |
| September | |||||
| Influent | + | − | − | 0 | 32 |
| Effluent | − | − | − | 0 | 23 |
a
Results obtained by nested PCR analysis of 4 ml of sewage for the detection of adenovirus or 2 ml for the detection of enterovirus or HAV. HAd, human adenovirus; EV, enterovirus.
b
Enterovirus PFU obtained from 20 ml of sewage; T, toxic effect.
c
Bacteriophage PFU obtained from 1 ml of sample.