Table 1.

List of primers used in current study.

Primers	Sequence (5′ → 3′)	Direction	Nucleotide
1st Round PCR
Outer forward	TTTCACCTCTGCCTAATCATCT	Forward	1823
Outer reverse	CAGACCAATTTATGCCTACAGCCT	Reverse	1801
2nd Round PCR
Forward (F1)	GGTCACCATATTCTTGGGAAC	Forward	2821
Reverse (R1)	TGAGAGAAGTCCACCACGAGT	Reverse	272
Forward (F2)	CTAGGACCCCTGCTCGTGTT	Forward	179
Reverse (R2)	CGAACCACTGAACAAATGGCACT	Reverse	704
Forward (F3)	GTATTCCCATCCCATCATCCTG	Forward	599
Reverse (R3)	GCTAGGAGTTCCGCAGTATGG	Reverse	1286