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. 2023 Nov 6;11(11):2717. doi: 10.3390/microorganisms11112717

Table 3.

Pairwise homoplasy index (PHI) test results for recombination of the Diaporthe arecae species complex based on concatenated and single gene sequence alignments of the 5-, 4- and 3-loci combined datasets.

Dataset Tested 1 Φw-Statistic (p-Value) 2
5-loci 4-loci 3-loci
ITS 0.19 (4.34 × 10−4) * 0.23 (0.01) * 0.27 (0.02) *
tef1 0.11 (0.02) * 0.13 (2.94 × 10−3) * 0.17 (1.12 × 10−3) *
tub2 0.10 (0.07) 0.12 (9.92 × 10−3) * 0.14 (0.23)
cal 0.20 (0.10) 0.19 (0.99) N/A
his3 0.13 (0.61) N/A N/A
Combined 0.16 (0.00) * 0.19 (0.00) * 0.22 (0.00) *

1 cal: partial calmodulin gene; his3: partial histone H3 gene; ITS: partial cluster of nrRNA genes, including the nuclear 5.8S rRNA gene and its flanking internally transcribed spacer regions ITS1 and ITS2; tef1: partial translation elongation factor 1-alpha gene; tub2: partial beta-tubulin gene; 2 5-loci: combined dataset based on ITS, tef1, tub2, cal and his3 loci; 4-loci: combined dataset based on ITS, tef1, tub2 and cal loci; 3-loci: combined dataset based on ITS, tef1 and tub2 loci; N/A: not applicable, locus excluded from the dataset; * Positive for recombination, PHI test yielded a p < 0.05.