Table 3.

Kinetic parameters and inhibitory constant, Ki, for hybrids IIf, IVb, and IVd.

		KM or KM app * (mM)	Vmax or Vmax app * (U.mg−1)	Inhibition Type and Ki *
Without inhibitor		0.4 ± 0.1	11.9 ± 2.3	-
IIf	5 µM	1.5	11.8	Competitive Ki = 6.2 µM
	20 µM	2.6	11.4
	40 µM	3.9	13.4
IVb	17.5 µM	0.57	7.5	Mixed (non-competitive) Ki = 30 µM
	35 µM	0.53	6.5
	70 µM	0.52	4.5
IVd	17.5 µM	1.0	9.6	Competitive Ki = 36 µM
	35 µM	1.6	9.0

* K_M—Michaelis constant; K_M ^app—apparent Michaelis constant; V_max—maximal velocity; V_max ^app—apparent maximal velocity; K_i—inhibitory constant.