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. Author manuscript; available in PMC: 2023 Nov 24.
Published in final edited form as: Cell Stem Cell. 2022 Sep 1;29(9):1366–1381.e9. doi: 10.1016/j.stem.2022.08.008

Figure 3. CYP8B1 overexpression potentiates colitis in mice, whereas CYP8B1 ablation ameliorates colitis.

Figure 3.

(A-H) AAV-Cyp8b1-OE and AAV-mCherry mice were treated with DSS for 5 days followed by 3 days of water (n = 5–8 mice/group). Body-weight loss (A), DAI (B), colon length (C), representative H&E staining and histology score of intestine sections (D), relative fluorescence intensity of FITC-dextran in serum (E), representative Muc2 or Lyz staining of intestine sections (F), Muc2 (G) and AMPs (H) mRNAs in intestine.

(I-P) AAV-shCyp8b1-KD and AAV-GFP mice were treated with DSS for 7 days followed by 3 days of water (n = 8–10 mice/group). Body-weight loss (I), DAI (J), colon length (K), representative H&E staining and histology score of intestine sections (L), relative fluorescence intensity of FITC-dextran in serum (M), representative Muc2 or Lyz staining of intestine sections (N), Muc2 (O) and AMPs (P) mRNAs in intestine.

(Q-X) Cyp8b1-null mice (Q-T, n = 9–10 mice/group) and Cyp8b1ΔHep mice (U-X, n = 7–8 mice/group) were treated with DSS for 7 days followed by 3 days of water. Body-weight loss (Q and U), DAI (R and V), colon length (S and W), representative H&E staining and histology score of intestine sections (T and X).

(D, F, L, N, T, X) Scale bars, 100 μm. (A-E, G-M, O-X) Mean ± SEM. Unpaired Student’s t-test. * P < 0.05; ** P < 0.01; *** P < 0.001. See also Figure S1–S3 and S5.