Fig. 3.

NIR-PIT enhanced Ti-DC migration to dLNs is inhibited by ATP-P2X7R, Gαi-protein blockade. Anti-CD44-IR700 or vehicle was intravenously administered 24 h before intratumoral administration of inhibitors at the time of NIR light and violet light irradiation of tumor and another 24 h later, cells in dLNs were analyzed. Number of KikGR-Red cells in total migratory DCs, and DC subsets in dLNs. Bar graph shows means ± SEM of pooled data from at least two independent experiments (n = 5–11). *P < 0.05, **P < 0.01, ***P < 0.001. (one-way ANOVA with Holm-Sidak’s multiple comparisons test)