TABLE 1.
Straina | Source (date [mo-yr]) | Country | Serovar Ee | Ribotypef | RAPD PCR resultg with primer:
|
|
---|---|---|---|---|---|---|
M13 | T3 | |||||
ATCC 27562T | Human wound infection | USAi | − | 1 | 4 | —h |
C7184b | Human blood | USA | − | 2 | 5 | 5 |
UMH1b | Fatal wound infection | USA | − | 3 | 1 | — |
Vv1b | Fatal wound infection | USA | − | 4 | 5 | 1 |
374b | Septicemia case | USA | − | 5 | 5 | 5 |
L180b | Septicemia case | USA | − | NDj | — | 7 |
TW1 | Tank water fish farm (10-90) | Spain | − | 6 | 1 | 5 |
LMG 12095 | Eel | Belgium | − | 6 | 8 | 7 |
M631 | Eel (9-90) | Spain | − | 7 | 2 | 2 |
D10 | Sea bream fish farm (7-90) | Spain | − | 8 | 6 | 7 |
M76 | Eel (7-90) | Spain | − | 8 | 6 | 7 |
E109 | Eel (7-90) | Spain | − | 8 | 6 | 7 |
E110 | Eel (7-90) | Spain | − | 8 | 6 | 7 |
M40 | Eel (3-90) | Spain | − | 9 | 1 | 3 |
M47 | Eel (3-90) | Spain | − | 9 | 1 | 3 |
M63 | Eel (3-90) | Spain | − | 9 | 1 | 3 |
M79 | Eel (3-90) | Spain | − | 9 | 1 | 3 |
M89 | Eel (3-90) | Spain | − | 9 | 1 | 3 |
M90 | Eel (3-90) | Spain | − | 9 | 1 | 3 |
M324 | Eel (3-90) | Spain | − | 9 | 1 | 3 |
M328 | Eel (3-90) | Spain | − | 9 | 1 | 3 |
M335 | Eel (3-90) | Spain | − | 9 | 1 | 3 |
M626 | Eel (9-90) | Spain | − | 9 | 1 | 1 |
M647 | Eel (9-90) | Spain | − | 9 | 2 | 1 |
E112 | Eel (9-90) | Spain | − | 9 | 2 | |
E113 | Eel (9-90) | Spain | − | 9 | 2 | |
E114 | Eel (9-90) | Spain | − | 9 | 1 | 1 |
P3 | Tank water fish farm (6-97) | Spain | − | 10 | 1 | 4 |
P4 | Tank water fish farm (6-97) | Spain | − | 10 | 1 | 4 |
P5 | Tank water fish farm (6-97) | Spain | − | 10 | 1 | 4 |
P6 | Tank water fish farm (6-97) | Spain | − | 10 | 1 | 4 |
143c | Eel | Belgium | − | 11 | 4 | 7 |
LMG 12090c | Eel | Belgium | − | 11 | 4 | 7 |
147c | Eel | Belgium | − | 11 | 4 | 7 |
149c | Eel | Belgium | − | 11 | 4 | 7 |
LMG 12092 | Eel | Belgium | − | 11 | 4 | 7 |
155c | Eel | Belgium | − | 11 | 4 | 7 |
161c | Eel | Belgium | − | 11 | 4 | 7 |
169c | Eel | Belgium | − | 11 | — | — |
160c | Eel | Belgium | − | 12 | 8 | 6 |
167c | Glass eel | Belgium | − | 13 | 9 | 1 |
248 | Eel | Belgium | − | 13 | 9 | 2 |
249c | Eel | Belgium | − | 13 | 9 | 2 |
170c | Human blood | Belgium | − | 14 | — | — |
LMG 13637 | Shrimp | Thailand | − | 15 | 8 | 6 |
VIB 537d | Shrimp | Thailand | − | 15 | — | 6 |
VIB 521d | Unknown | Unknown | − | 16 | — | 7 |
VIB 535d | Eel | Sweden | − | 17 | — | — |
VIB 536d | Eel | Sweden | − | 17 | — | 7 |
C1 | Seawater (4-96) | Spain | − | 18 | 7 | 7 |
C2 | Seawater (4-96) | Spain | − | 18 | 7 | 7 |
C32 | Seawater (12-96) | Spain | − | 18 | 7 | 7 |
C33 | Seawater (12-96) | Spain | − | 18 | 7 | 7 |
C36 | Seawater (5-97) | Spain | − | 18 | 6 | 7 |
C37 | Seawater (5-97) | Spain | − | 18 | 6 | 7 |
C38 | Seawater (5-97) | Spain | − | 18 | 6 | 7 |
C39 | Seawater (5-97) | Spain | − | 18 | 6 | 7 |
C3 | Seawater (4-96) | Spain | − | 19 | 1 | 4 |
C4–C25 | Wedge shell (5-96) | Spain | − | 20 | 1 | 7 |
C26 | Seawater (5-96) | Spain | − | 21 | 11 | 12 |
C27 | Seawater (5-96) | Spain | − | 22 | 11 | — |
C28 | Seawater (5-96) | Spain | − | 22 | — | — |
C29 | Seawater (5-96) | Spain | − | 23 | 13 | 12 |
C30 | Seawater (5-96) | Spain | − | 23 | 13 | 12 |
C31 | Seawater (5-96) | Spain | − | 24 | 7 | 7 |
C34 | Seawater (5-97) | Spain | − | 25 | 12 | 9 |
C35 | Seawater (5-97) | Spain | − | 25 | 12 | 9 |
C40 | Wedge shell (5-97) | Spain | − | 26 | — | — |
C41 | Seawater (6-97) | Spain | − | 27 | 10 | 11 |
C42 | Seawater (6-97) | Spain | − | 28 | 10 | 10 |
C43 | Common littleneck (6-97) | Spain | − | ND | 10 | 11 |
C44 | Common littleneck (8-97) | Spain | − | 29 | 10 | — |
C45 | Wedge shell (8-97) | Spain | − | 30 | 10 | 10 |
C46 | Wedge shell (8-97) | Spain | − | 31 | 5 | 7 |
C47 | Wedge shell (8-97) | Spain | − | 31 | 5 | 7 |
ATCC 33149 | Diseased eel | Japan | + | 32 | 3 | 8 |
NCIMB 2136 | Diseased eel | Japan | + | 32 | 3 | 8 |
NCIMB 2137 | Diseased eel | Japan | + | 32 | 3 | 8 |
E4 | Diseased eel (89) | Spain | + | 32 | 3 | 8 |
E12 | Diseased eel (89) | Spain | + | 32 | 3 | 8 |
E22 | Diseased eel (89) | Spain | + | 32 | 3 | 8 |
E24 | Diseased eel (89) | Spain | + | 32 | 3 | 8 |
E32 | Diseased eel (89) | Spain | + | 32 | 3 | 8 |
E37 | Diseased eel (89) | Spain | + | 32 | 3 | 8 |
E39 | Diseased eel (89) | Spain | + | 32 | 3 | 8 |
E40 | Diseased eel (89) | Spain | + | 32 | 3 | 8 |
E52 | Diseased eel (89) | Spain | + | 32 | 3 | 8 |
E56 | Diseased eel (89) | Spain | + | 32 | 3 | 8 |
E58 | Diseased eel (89) | Spain | + | 32 | 3 | 8 |
E64 | Diseased eel (90) | Spain | + | 32 | 3 | 8 |
E80 | Diseased eel (90) | Spain | + | 32 | 3 | 8 |
E86 | Diseased eel (90) | Spain | + | 32 | 3 | 8 |
E92 | Diseased eel (90) | Spain | + | 32 | 3 | 8 |
M206 | Diseased eel (90) | Spain | + | 32 | 3 | 8 |
M338 | Diseased eel (90) | Spain | + | 32 | 3 | 8 |
E103 | Diseased eel (90) | Spain | + | 32 | 3 | 8 |
E105 | Diseased eel (90) | Spain | + | 32 | 3 | 8 |
E106 | Diseased eel (90) | Spain | + | 32 | 3 | 8 |
ER1 | Diseased eel (92) | Spain | + | 32 | 3 | 8 |
R1 | Diseased eel (94) | Spain | + | 32 | 3 | 8 |
R2 | Diseased eel (94) | Spain | + | 32 | 3 | 8 |
VIB 525d | Diseased eel | Sweden | + | 32 | 3 | 8 |
VIB 526d | Diseased eel | Sweden | + | 32 | 3 | 8 |
VIB 527d | Diseased eel | Sweden | + | 32 | 3 | 8 |
VIB 524d | Diseased eel | Norway | + | 32 | 3 | 8 |
171c | Diseased eel | Belgium | + | 32 | — | 7 |
VIB 523d | Diseased eel | Sweden | + | 32 | — | 7 |
NCIMB 2138 | Diseased eel | Japan | + | 33 | 3 | 8 |
LMG 13638 | Shrimp | Unknown | + | 33 | 3 | 8 |
A1 | Diseased eel (97) | Spain | + | 33 | 3 | 8 |
VIB 522d | Human blood | Unknown | + | 34 | 3 | 8 |
ATCC, American Type Culture Collection, Manassas, Va.; NCIMB, National Collection of Industrial and Marine Bacteria, Aberdeen, Scotland; LMG, Laboratorium voor Microbiologie, Rijksuniversiteit, Ghent, Belgium. Strains in boldface correspond to the new V. vulnificus isolates (C1 to C47 are Mediterranean isolates; P3 to P6 and A1 are new eel farm isolates).
Supplied by J. D. Oliver, University of North Carolina, Charlotte.
Supplied by L. Grisez, Katholieke Universiteit, Leuven, Belgium.
Supplied by L. Verdonck, Laboratorium voor Microbiologie, Rijksuniversiteit, Ghent, Belgium.
Ascription to serovar E was performed by slide agglutination with serum anti-E39 (diseased-eel isolate). +, serovar E; −, not serovar E.
Ribotype designations are each preceded by RT (e.g., RT 1).
Clusters obtained by RAPD PCR after the UPGMA analysis.
—, ungrouped strain.
USA, United States.
ND, not determined.