Fig. 5. FimI anchors type 1 pili to E. coli cells.

a Yeast agglutination assays performed with E. coli W3110, W3110ΔfimI and W3110ΔfimA, which had been grown under shaking or static conditions and diluted to OD₆₀₀ = 1.0 prior to mixing with the yeast cells. To complement the fimI deletion, the plasmid pKTCTET-IC was used and gene expression induced with 30 ng/ml anhydrotetracycline (aTc). Mannose specificity of yeast cell binding was tested by a competition assay in the presence of 1 M D-mannose or 1 M D-glucose (negative control). b Yeast agglutination assay with E. coli W3110 and W3110ΔfimI, grown under shaking conditions and diluted to the indicated OD₆₀₀ values prior to mixing with the yeast cells.