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. 2023 Nov 24;14:7718. doi: 10.1038/s41467-023-43449-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — For determination of the FimC_His dissociation rate constant (k_off), 4 µM of FimC_HisI (a), FimC_HisIA (b), FimC_HisFt (c) or FimC_HisFG (d) were incubated with a 9-fold molar excess of FimC. Relaxation to the new equilibrium was monitored by analytical cation exchange chromatography. Solid lines are fits according to a single-exponential function. Source data are provided as a Source Data file.