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. 2023 Nov 24;14:7692. doi: 10.1038/s41467-023-43538-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Left: SDS-PAGE of isolated bovine cardiac myosin S1 with essential light chain (ELC) and myosin heavy chain (MHC) labeled accordingly. Middle: Representative traces of mant-ATP chase experiments in the presence of vehicle control (gray), 2 μmol L^-1 Mavacamten (purple) or 20 μmol L^-1 F10 (red). Bi-exponential fits to data are shown as black continuous lines. Right: Fraction of slow phase nucleotide release for the three conditions. Means ± s.e.m., n = 5 independent repeats for control, and n = 6 independent repeats in the presence of F10 or Mavacamten. b Left: SDS-PAGE of isolated bovine cardiac myosin with regulatory light chain (RLC), essential light chain (ELC) and myosin heavy chain (MHC) labeled accordingly. Middle: Representative traces of mant-ATP chase experiments in the presence of vehicle control (gray), Mavacamten (purple) and F10 (red). Bi-exponential fits to data are shown as black continuous lines. Right: Fraction of slow phase nucleotide release. Means ± s.e.m., n = 5 independent repeats for control, and n = 7 independent repeats in the presence of F10 or Mavacamten. c Left: SDS-PAGE of isolated bovine cardiac myofibrils with main proteins labeled accordingly (MHC – myosin heavy chain, MyC – myosin binding protein-C, Tm – tropomyosin, TnI – troponin I, ELC – essential light chain, RLC – regulatory light chain, TnC – troponin C). Middle: Representative traces of mant-ATP chase experiments in the presence of vehicle control (gray), 2 μmol L⁻¹ Mavacamten (purple) or 20 μmol L⁻¹ F10 (red). Bi-exponential fits to data are shown as black continuous lines. Right: Fraction of slow phase nucleotide release for the three conditions. Means ± s.e.m., n = 5 independent repeats for control, n = 6 independent repeats in the presence of F10 and n = 6 independent repeats in the presence of Mavacamten. Statistical significance of differences between groups were assessed with a one-way ANOVA followed by Tukey’s post-hoc test. Source data are provided as a Source Data file.