Figure 3.

Follicular fluid (FF) has dose-dependent effects on oocyte spindle morphology. (A) Representative z-projections of metaphase II spindle length (white bracket) and spindle pole width (red bracket) for eggs following maturation without FF (control) or in 100% FF. Actin (rhodamine phalloidin, red), pericentrin (magenta), α-tubulin (green), and DNA (DAPI, blue) were detected by immunocytochemistry. Scale bars = 10 μm. (B–D) Quantification of spindle length (B), average pole width (C), and average pole width: spindle length ratio (D) for eggs following maturation in different concentrations of FF. Data are shown as mean±SEM. **P-value ≤0.01 by one-way ANOVA with multiple comparisons. N = 4–29 eggs per group across total of three replicates.