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. 2023 Nov 9;29(11):gaad039. doi: 10.1093/molehr/gaad039

Figure 7.

Figure 7.

Exposure of intact cumulus–oocyte complexes (COCs) to follicular fluid (FF) has age-dependent effects on meiotic progression, but not spindle morphology. (A) Graph of meiotic progression for COCs from young (i) and old (ii) mice matured without FF (control), in young FF, or in old FF. Data are shown as mean±SEM for all panels. N = 29–56 COCs per group across total of four trials. (i) **P = 0.0015 for young COCs that extruded polar bodies (PBE) versus no PBE by Fisher’s exact test. (ii) *P = 0.0259 for old COCs PBE versus no PBE by Fisher’s exact test. (B) Quantification of abnormal phenotypes of metaphase II eggs from COCs from young (i) and old (ii) mice following maturation without FF (control), in young FF, or in old FF. N = 24–55 COCs per group across total of four trials. (C–E) Quantification of spindle length (C), average pole width (D), and average pole width: spindle length ratio (E) for COCs from young (i) and old (ii) mice matured without FF (control), in young FF, or in old FF. N = 18–40 COCs per group across total of four trials.