Figure 1.

Pteropodid TRIM40 is an interferon-stimulated gene. (A,B) Transfection of A549 (human lung), PVK4 (Pteropus vamyprus kidney), and ZFBK13-75 (Eidolon helvum kidney) cells with 1 μg/mL of low-molecular weight (LMW) poly(I:C). (A) Immunoblots with antibodies specific to interferon induction regulators (pTBK1 and pSTAT1) and interferon-stimulated genes (total STAT1 and RIG-I). (B) At four hours post-stimulation, RNA was collected for quantitative PCR of the TRIM40 gene. Fold induction of poly(I:C)-stimulated samples relative to mock-transfected samples is shown. (C,D) A549, PVK4, and ZFBK13-75 cells were stimulated with 500 U/mL of universal type I interferon (U-IFN). (C) Cell lysates were collected for Western blot to probe for activated STAT1 (pSTAT1 Y701) and STAT2 (pSTAT2 Y690) as well as their total protein levels. (D) At four hours post-stimulation, RNA was collected for qPCR, and log₂ fold change is presented for canonical ISGs (Mx1, OAS1, TRIM25, and TRIM56), non-IFN-regulated TRIM23, and the gene of interest, TRIM40.