Skip to main content
. 2023 Oct 25;15(11):2147. doi: 10.3390/v15112147

Figure 2.

Figure 2

Pteropus vampyrus TRIM40 antagonizes type I interferon and pro-inflammatory cytokine gene expression. siRNA targeting Pteropus vampyrus TRIM40 or scrambled control was transfected in PVK4 cells 48 h prior to the transfection of 1 μg/mL of low-molecular weight (LMW) poly(I:C). (A) The RNA collected at 24 h post-poly(I:C) transfection shows knockdown efficiency. (B) Lysates collected in laemmli were used for Western blot to measure activated (pSTAT2) and total STAT2. (C) RNA from lysates was used to measure fold-induction of ISGs (RIG-I, ISG56, Mx1, and OAS1) and pro-inflammatory cytokines (IL6 and TNFα). p values are reported as * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001.