TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant phenotype | Source or reference |
|---|---|---|
| Strains | ||
| C. acidovorans DS-17 (JCM10181) | Wild type | 31 |
| A. eutrophus PHB−4 (DSM541) | PHA-negative mutant of wild-type H16 | 35 |
| E. coli | ||
| S17-1 | recA and tra genes of plasmid RP4 integrated into the chromosome; auxotrophic for proline and thiamine | 40 |
| DH5α | deoR endA1 gyrA96 hsdR17(rK− mK+) recA1 relA1 supE44 thi-1 Δ(lacZYA-argFV169) φ80ΔlacZΔM15 F− λ− | Clontech |
| Plasmids | ||
| pLA2917 | Cosmid; Kmr Tcr | 1 |
| pLACa | pLA2917 derivative; 20-kbp gDNA fragment | This study |
| pJRD215 | Cosmid; Kmr Smr RSF1010 replicon; Mob+ | 3 |
| pJRDEV80 | pJRD215 derivative; phaCCa phaACa | This study |
| pBluescript II KS(+) | AprlacPOZ; T7 and T3 promoter | Stratagene |
| pEV80 | pBluescript derivative; phaCCa phaACa | This study |