TABLE 2.
PHA accumulation by A. eutrophus PHB−4 complemented with pLACa and by wild types of A. eutrophus and C. acidovoransa
| Bacterium and carbon source | Dry cell wt (g/liter) | PHA content (wt%)b | PHA composition (mol%)
|
||
|---|---|---|---|---|---|
| 3HB | 3HV | 4HB | |||
| A. eutrophus PHB−4(pLACa) | |||||
| Fructose | 4.2 | 73 | 100 | 0 | 0 |
| Pentanoate | 1.8 | 27 | 56 | 44 | 0 |
| 4HB | 1.5 | 5 | 86 | 0 | 14 |
| 1,4-Butanediol | 1.3 | 2 | 100 | 0 | 0 |
| A. eutrophus H16 | |||||
| Fructose | 4.3 | 72 | 100 | 0 | 0 |
| Pentanoate | 2.0 | 65 | 25 | 75 | 0 |
| 4HB | 1.3 | 16 | 66 | 0 | 34 |
| 1,4-Butanediol | 1.2 | 22 | 89 | 0 | 11 |
| C. acidovorans DS-17 | |||||
| Fructose | 2.4 | 7 | 100 | 0 | 0 |
| Pentanoate | 2.4 | 43 | 39 | 61 | 0 |
| 4HB | 2.2 | 31 | 0 | 0 | 100 |
| 1,4-Butanediol | 2.1 | 17 | 3 | 0 | 97 |
a
Cells were cultivated in 100 ml of NR medium for 12 h and then transferred into nitrogen-free MS medium containing 1% (wt/vol) carbon source for 48 h at 30°C.
b
Percent of the dry cell weight.