Figure 6.

Assessment of cytokine expression using RT-qPCR. BHK-21 cells were infected at an MOI of 0.2 in the absence or presence of different concentrations of FF72 for 24 h. At the end of this period, total RNA was extracted, and expression levels of IL-1β (A), IL-6 (B), IL-8 (C), and MCP-1 (D) were determined using qRT-PCR. Statistical significance was calculated using a nonparametric Student t test, in which * represents p values lower than 0.05 compared to vehicle-treated condition.