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. 2023 Oct 31;15(11):2190. doi: 10.3390/v15112190

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Producing an infectious clone of Zika virus (ZIKV) with teLuc, a version of the nanoluciferase gene with improved spectral properties. (a) Schematic representation of the genetic composition of teLuc-ZIKV infectious clone. Red vertical lines represent the position of the primers used for cloning. (b) Size and purity of amplicons generated during cloning. Agarose gel (1.2%) Lane 1: 1 kb DNA ladder; Lane 2: 100 bp DNA ladder; Lane 3: Amplicon 3; Lane 4: Amplicon 1; Lane5: Amplicon 2. The size and mass of the DNA ladders loaded in Lanes 1 (c) and 2 (d).