Figure 2.

Titration and infection kinetics of reporter ZIKV in Vero cells. (a) Kinetics of viral growth for nLuc-ZIKV in Vero cells at three different dilutions, 1:10³ (●), 1:3 × 10³ (■), and 1:10⁴ (▲), represented as luminescence arbitrary units (AU). Means of four replicates and error bars representing standard deviations are shown. The experiment was performed once. (b) Titration of nLuc- and teLuc-ZIKV in Vero cells. Serial dilutions of teLuc- and nLuc-ZIKV in quadruplicates were added onto Vero cells for 48 h, and then media removed, and luciferase activity measured. Means from four repeats with error bars representing standard deviations are shown.