Table 1.

Sequences of the primers and annealing temperatures for the real-time PCR analysis of the IGF1 and GAPDH genes.

Gene	Primer Sense (Forward)	Primer Antisense (Reverse)
IGF1	5′-AAGCCTACAAAGTCAGCTCG- 3′	5′-GGTCTTGTTTCCTGCACTT-3′
GAPDH	5′-GATGGGTGTGAACCACGAGAAA-3′	5′-ACGGATACATTGGGGGTAGGA-3′

Abbreviations: IGF1, insulin-like growth factor 1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.