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Immunoblot analysis of the BoHV-1qmv Sub-RVFV-expressed chimeric Gn-GMCSF and Gc proteins for glycosylation. KOP-R cells were infected with the BoHV-1qmv Sub-RVFV. Infected cell lysates were immunoprecipitated with either (A) RVFV Gn- or (B) RVFV Gc-specific antibodies. Immunoprecipitated proteins were either untreated (−) or treated with PNGase F (+) or Endo H (+) and subjected to SDS-PAGE and immunoblotting with FLAG tag (Gn-specific) or V5 tag (Gc-specific) antibodies.
